Proteomic analyses showed that known oxidative stress defense proteins are induced in the trxB1 mutant. Aerobic growth of the trxB1 mutant did not require glutathione, also ruling out the need for this redox maintenance system. ![]() Unexpectedly, the trxB1 mutant was viable without DTT and under aerated static conditions, thus disproving the essentiality of this system. The mutant was obtained under anaerobic conditions in the presence of dithiothreitol (DTT). ![]() Some gram-positive bacteria, including Lactococcus lactis, do not produce glutathione, and the thioredoxin system is presumed to be essential. Thiol-disulfide bond balance is generally maintained in bacteria by thioredoxin reductase-thioredoxin and/or glutathione-glutaredoxin systems.
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